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PLoS One. 2014 May 14;9(5):e96895. doi: 10.1371/journal.pone.0096895. eCollection 2014.

Proteomics of vitreous humor of patients with exudative age-related macular degeneration.

Author information

1
Department of Ophthalmology, Goethe University, Frankfurt am Main, Germany; Doheny Eye Institute, Los Angeles, California, United States of America; Department of Ophthalmology, Ruprecht Karls University, Heidelberg, Germany.
2
Mosaiques Diagnostics, Hannover, Germany.
3
Department of Ophthalmology, Goethe University, Frankfurt am Main, Germany.
4
Doheny Eye Institute, Los Angeles, California, United States of America.
5
Mosaiques Diagnostics, Hannover, Germany; BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, United Kingdom.
6
BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, United Kingdom.
7
Department of General Ophthalmology, Lublin University, Poland.
8
Department of Nephrology, Endocrinology, and Transplantation Medicine Charité-Universitaetsmedizin, Berlin, Germany.
9
Department of Ophthalmology, Philipps University, Marburg, Germany.
10
Mosaiques Diagnostics, Hannover, Germany; Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, Toulouse, France; Université Toulouse III Paul-Sabatier, Toulouse, France.
11
Mosaiques Diagnostics, Hannover, Germany; Department of Nephrology, Endocrinology, and Transplantation Medicine Charité-Universitaetsmedizin, Berlin, Germany.

Abstract

BACKGROUND:

There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD).

METHODS AND FINDINGS:

Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity.

CONCLUSIONS:

Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD.

PMID:
24828575
PMCID:
PMC4020801
DOI:
10.1371/journal.pone.0096895
[Indexed for MEDLINE]
Free PMC Article

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