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Sci Rep. 2014 May 14;4:4954. doi: 10.1038/srep04954.

Human leukocyte telomere length is associated with DNA methylation levels in multiple subtelomeric and imprinted loci.

Author information

1
1] Section of Investigative Medicine, Department of Medicine, Imperial College London, London W12 0NN, UK [2].
2
1] University of Bristol, Bristol, UK [2] McGill University, Montreal, Quebec, Canada [3].
3
1] McGill University, Montreal, Quebec, Canada [2] University of Toronto, Toronto, Ontario, Canada.
4
Lebanese International University and Lebanese University, Beirut, Lebanon.
5
University of Bristol, Bristol, UK.
6
Section of Investigative Medicine, Department of Medicine, Imperial College London, London W12 0NN, UK.
7
1] University of British Columbia, Vancouver, British Columbia, Canada [2].
8
UCL Institute of Child Health, London, UK.
9
McGill University, Montreal, Quebec, Canada.

Abstract

In humans, leukocyte telomere length (LTL) is positively correlated with lifespan, and shorter LTL is associated with increased risk of age-related disease. In this study we tested for association between telomere length and methylated cytosine levels. Measurements of mean telomere length and DNA methylation at >450,000 CpG sites were obtained for both blood (N = 24) and EBV-transformed cell-line (N = 36) DNA samples from men aged 44-45 years. We identified 65 gene promoters enriched for CpG sites at which methylation levels are associated with leukocyte telomere length, and 36 gene promoters enriched for CpG sites at which methylation levels are associated with telomere length in DNA from EBV-transformed cell-lines. We observed significant enrichment of positively associated methylated CpG sites in subtelomeric loci (within 4 Mb of the telomere) (P < 0.01), and also at loci in imprinted regions (P < 0.001). Our results pave the way for further investigations to help elucidate the relationships between telomere length, DNA methylation and gene expression in health and disease.

PMID:
24828261
PMCID:
PMC4344300
DOI:
10.1038/srep04954
[Indexed for MEDLINE]
Free PMC Article

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