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J Am Assoc Lab Anim Sci. 2014 May;53(3):228-31.

Transcervical embryo transfer in mice.

Author information

1
College of Animal Science and Technology, Hebei University, Baoding, China.
2
School Hospital of Agricultural University of Hebei, Hebei University, Baoding, China.
3
Centre of Laboratory Animal, Faculty of Medicine, Hebei University, Baoding, China.
4
College of Animal Science and Technology, Hebei University, Baoding, China. Lxyun@hebau.edu.cn.

Abstract

Although efficient transcervical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of transcervical transfer has hampered its acceptance and use. Here, we describe a novel device and protocol for transcervical embryo transfer in mice. Blastocysts from CD1 female mice were transferred into the uteri of 2.5-d pseudopregnant CD1 mice by using this method resulted in the successful development of 66.7% to 73.5% of the transferred blastocysts into live-born fetuses. Our method is as efficient as surgical embryo transfer yet is much simpler, easier, and markedly less traumatic to the recipient. In addition, our method provides hygienic and economic advantages and conforms to the principles of humane experimental technique. More importantly, our method provides a model for studying transcervical embryo transfer in cattle, other large animals, and humans.

PMID:
24827563
PMCID:
PMC4128559
[Indexed for MEDLINE]
Free PMC Article
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