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Proteomics. 2014 Oct;14(19):2190-9. doi: 10.1002/pmic.201400060. Epub 2014 Jul 9.

Site-specific human histone H3 methylation stability: fast K4me3 turnover.

Author information

1
Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, 60208, USA.

Abstract

We employ stable-isotope labeling and quantitative mass spectrometry to track histone methylation stability. We show that H3 trimethyl K9 and K27 are slow to be established on new histones and slow to disappear from old histones, with half-lives of multiple cell divisions. By contrast, the transcription-associated marks K4me3 and K36me3 turn over far more rapidly, with half-lives of 6.8 h and 57 h, respectively. Inhibition of demethylases increases K9 and K36 methylation, with K9 showing the largest and most robust increase. We interpret different turnover rates in light of genome-wide localization data and transcription-dependent nucleosome rearrangements proximal to the transcription start site.

KEYWORDS:

Cell biology; Chromatin; Demethylation; Heterochromatin; Methylation turnover; SILAC; Transcription

PMID:
24826939
PMCID:
PMC4184988
DOI:
10.1002/pmic.201400060
[Indexed for MEDLINE]
Free PMC Article

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