Format

Send to

Choose Destination
J Invest Dermatol. 2014 Nov;134(11):2747-2756. doi: 10.1038/jid.2014.221. Epub 2014 May 12.

Propionibacterium acnes activates the NLRP3 inflammasome in human sebocytes.

Author information

1
Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.
2
Department of Anatomy, College of Medicine, Chungnam National University, Daejeon, Korea.
3
Department of Dermatology, The Affiliated hospital of Guangdong Medical College, Zhanjiang, China.
4
Department of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.
5
Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea. Electronic address: im1177@hanmail.net.

Abstract

Propionibacterium acne and sebaceous glands are considered to have an important role in the development of acne. Although information regarding the activation of innate immunity by P. acnes in the sebaceous gland is limited, different P. acnes phylotypes and a higher prevalence of follicular P. acnes macrocolonies/biofilms in sebaceous follicles of skin biopsies from acne compared with control skin and occasionally single P. acnes clusters in single sebaceous glands have been detected. In this study, we investigated whether P. acnes activates the inflammasome in human sebaceous glands in vivo and in vitro. We found that IL-1β expression was upregulated in sebaceous glands of acne lesions. After stimulation of human sebocytes with P. acnes, the activation of caspase-1 and secretion of IL-1β were enhanced significantly. Moreover, knocking down the expression of NLRP3 abolished P. acnes-induced IL-1β production in sebocytes. The activation of the NLRP3 inflammasome by P. acnes was dependent on protease activity and reactive oxygen species generation. Finally, we found that NALP3-deficient mice display an impaired inflammatory response to P. acnes. These results suggest that human sebocytes are important immunocompetent cells that induce the NLRP3 inflammasome, and that P. acnes-induced IL-1β activation in sebaceous glands may have a role in combating skin infections and in acne pathogenesis.

PMID:
24820890
DOI:
10.1038/jid.2014.221
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center