Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16.

Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.

Author information

1
1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA. [3] Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark.
2
1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA.
3
TransgeneOmics, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
4
Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
5
Department of Biology, University of Utah, Salt Lake City, Utah, USA.

Abstract

We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.

PMID:
24820376
PMCID:
PMC4126194
DOI:
10.1038/nmeth.2889
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center