The disinfection of impression materials by using microwave irradiation and hydrogen peroxide

J Prosthet Dent. 2014 Oct;112(4):981-7. doi: 10.1016/j.prosdent.2013.12.017. Epub 2014 May 10.

Abstract

Statement of problem: Microwave irradiation and immersion in solutions have been recommended for denture disinfection. However, the effect of dry conditions and impression materials has not been completely evaluated.

Purpose: The purpose of this study was to evaluate the effectiveness of microwave irradiation and hydrogen peroxide for the disinfection of dental impression materials.

Material and methods: Specimens (diameter 10 mm, thickness 2 mm) were made with polyvinyl siloxane. Experimental groups were treated with hydrogen peroxide (group H), microwave irradiation (group M), and a combination of both hydrogen peroxide and microwave irradiation (group MH) for 1 minute, 2 minutes, and 3 minutes. The control group was untreated. The total sample size was 120. The specimens were divided into 2 groups, those exposed to Streptococcus mutans and those exposed to Escherichia coli. The disinfection effect and physical properties (contact angle, compatibility with gypsum, strain in compression, tear strength) were evaluated.

Results: All 3 groups (H, M, MH) were effective in reducing the number of colony forming units (CFU) per unit volume (mL) for both S mutans and E coli compared with the control. The most significant reduction in the CFU/mL of both bacteria was noted in the MH group and was used to compare either treatment alone (P<.05). No statistically significant difference was noted between the control and treatment groups in terms of all of the physical properties tested (P>.05).

Conclusions: Microwave irradiation was identified as a useful disinfection method against S mutans and E coli, especially when combined with H2O2, without adversely affecting the physical properties of dental impression materials.

Publication types

  • Comparative Study

MeSH terms

  • Bacterial Load / drug effects
  • Bacterial Load / radiation effects
  • Bacteriological Techniques
  • Calcium Sulfate / chemistry
  • Dental Disinfectants / therapeutic use*
  • Dental Impression Materials / chemistry*
  • Dental Impression Materials / radiation effects
  • Dental Stress Analysis / instrumentation
  • Disinfection / methods*
  • Escherichia coli / drug effects
  • Escherichia coli / radiation effects
  • Humans
  • Hydrogen Peroxide / therapeutic use*
  • Materials Testing
  • Microwaves / therapeutic use*
  • Polyvinyls / chemistry
  • Polyvinyls / radiation effects
  • Radiation Dosage
  • Siloxanes / chemistry
  • Siloxanes / radiation effects
  • Streptococcus mutans / drug effects
  • Streptococcus mutans / radiation effects
  • Stress, Mechanical
  • Surface Properties
  • Temperature
  • Tensile Strength
  • Time Factors
  • Wettability

Substances

  • Dental Disinfectants
  • Dental Impression Materials
  • Polyvinyls
  • Siloxanes
  • vinyl polysiloxane
  • Hydrogen Peroxide
  • Calcium Sulfate