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Methods Mol Biol. 2014;1149:271-9. doi: 10.1007/978-1-4939-0473-0_22.

LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP.

Author information

1
Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.

Abstract

The secondary messenger molecule, 3',5'-cyclic diguanosine monophosphate (c-di-GMP), controls various cellular processes in bacteria. Direct measurement of intracellular concentration of c-di-GMP is fast becoming an important tool for studying prokaryotic biology. Here, we describe a comprehensive extraction protocol from live bacteria and quantitative analysis using LC/MS/MS.

PMID:
24818912
DOI:
10.1007/978-1-4939-0473-0_22
[Indexed for MEDLINE]

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