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Methods Mol Biol. 2014;1149:59-65. doi: 10.1007/978-1-4939-0473-0_7.

Plate-based assay for swimming motility in Pseudomonas aeruginosa.

Author information

1
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Abstract

Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.

PMID:
24818897
DOI:
10.1007/978-1-4939-0473-0_7
[Indexed for MEDLINE]

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