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J Photochem Photobiol B. 2014 Jul 5;136:12-8. doi: 10.1016/j.jphotobiol.2014.04.007. Epub 2014 Apr 20.

Protective effects of citrus and rosemary extracts on UV-induced damage in skin cell model and human volunteers.

Author information

1
Skin Research Platform (SRP), Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Avenida de la Universidad s/n, E-03202 Elche, Alicante, Spain.
2
Monteloeder S.L., Miguel Servet 16, nave 17, 03203 Elche, Alicante, Spain.
3
Nutrafur S.A., Camino Viejo de Pliego, km.2, 30820 Alcantarilla, Murcia, Spain; Institute of Research Into Aging, University of Murcia, 30100 Espinardo, Murcia, Spain.
4
Radiology and Physical Medicine Department, Faculty of Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain; Institute of Research Into Aging, University of Murcia, 30100 Espinardo, Murcia, Spain.
5
Skin Research Platform (SRP), Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Avenida de la Universidad s/n, E-03202 Elche, Alicante, Spain. Electronic address: vmicol@umh.es.

Abstract

Ultraviolet radiation absorbed by the epidermis is the major cause of various cutaneous disorders, including photoaging and skin cancers. Although topical sunscreens may offer proper skin protection, dietary plant compounds may significantly contribute to lifelong protection of skin health, especially when unconsciously sun UV exposed. A combination of rosemary and citrus bioflavonoids extracts was used to inhibit UV harmful effects on human HaCaT keratinocytes and in human volunteers after oral intake. Survival of HaCaT cells after UVB radiation was higher in treatments using the combination of extracts than in those performed with individual extracts, indicating potential synergic effects. The combination of extracts also decreased UVB-induced intracellular radical oxygen species (ROS) and prevented DNA damage in HaCaT cells by comet assay and decreased chromosomal aberrations in X-irradiated human lymphocytes. The oral daily consumption of 250 mg of the combination by human volunteers revealed a significant minimal erythema dose (MED) increase after eight weeks (34%, p<0.05). Stronger protection was achieved after 12 weeks (56%, p<0.01). The combination of citrus flavonoids and rosemary polyphenols and diterpenes may be considered as an ingredient for oral photoprotection. Their mechanism of action may deserve further attention.

KEYWORDS:

Citrus; DNA damage; Oral photoprotection; Polyphenols; ROS; Rosemary; UV radiation

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