Format

Send to

Choose Destination
J Gen Virol. 2014 Aug;95(Pt 8):1634-9. doi: 10.1099/vir.0.065201-0. Epub 2014 May 9.

Pseudotyping of vesicular stomatitis virus with the envelope glycoproteins of highly pathogenic avian influenza viruses.

Author information

1
Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland gert.zimmer@ivi.admin.ch.
2
Institut für Virologie und Immunologie (IVI), Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland.

Abstract

Pseudotype viruses are useful for studying the envelope proteins of harmful viruses. This work describes the pseudotyping of vesicular stomatitis virus (VSV) with the envelope glycoproteins of highly pathogenic avian influenza viruses. VSV lacking the homotypic glycoprotein (G) gene (VSVΔG) was used to express haemagglutinin (HA), neuraminidase (NA) or the combination of both. Propagation-competent pseudotype viruses were only obtained when HA and NA were expressed from the same vector genome. Pseudotype viruses containing HA from different H5 clades were neutralized specifically by immune sera directed against the corresponding clade. Fast and sensitive reading of test results was achieved by vector-mediated expression of GFP. Pseudotype viruses expressing a mutant VSV matrix protein showed restricted spread in IFN-competent cells. This pseudotype system will facilitate the detection of neutralizing antibodies against virulent influenza viruses, circumventing the need for high-level biosafety containment.

PMID:
24814925
DOI:
10.1099/vir.0.065201-0
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center