Format

Send to

Choose Destination
Int J Pediatr Otorhinolaryngol. 2014 Jul;78(7):995-9. doi: 10.1016/j.ijporl.2014.03.013. Epub 2014 Mar 27.

Observation of permeability of blood-labyrinth barrier during cytomegalovirus-induced hearing loss.

Author information

1
Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China.
2
Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China.
3
Department of Otolaryngology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, China; Institute of Audiology and Speech Science of Xuzhou Medical College, Xuzhou 221006, China. Electronic address: oto8558@163.com.
4
Laboratory of Transplantation Immunity of Xuzhou Medical College, Xuzhou 221006, China.
5
School of Basic Education Science of Xuzhou Medical College, Xuzhou 221006, China.

Abstract

OBJECTIVE:

Congenital cytomegalovirus (CMV) infection is the most common infectious cause of sensorineural hearing loss in children. This study aims to investigate the pathogenesis CMV-induced hearing loss from the view of integrity of blood-labyrinth-barrier (BLB).

METHODS:

Newborn BALB/c mice were randomly divided into three groups (n=22, respectively): CMV group, control group and normal group. The CMV group and control group were intracerebrally injected with equal volume (15 μl) of murine CMV (MCMV; 10(4)IU/0.1 ml) and PBS, respectively, and normal group did not receive any treatment. After three weeks, auditory-evoked brainstem response was assessed, and permeability of BLB was evaluated by Evans blue method. Means between groups were compared using t-test.

RESULTS:

We observed that mice injected with MCMV had a hearing loss and it was connected with the permeability changes of BLB. Besides, using hematoxylin-eosin staining, we noticed hyperaemia in stria vascularis and spiral ligament and bleeding in scala vestibule and scala tympani in CMV group.

CONCLUSION:

All these data indicated the possible association between CMV-induced hearing loss and BLB dysfunction with the characteristics of inflammation. Our data provide a possible path to investigate the mechanism of CMV-induced hearing damage.

KEYWORDS:

Blood–labyrinth-barrier; Cytomegalovirus; Inflammation; Permeability

PMID:
24814236
DOI:
10.1016/j.ijporl.2014.03.013
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center