Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2014 Jun 27;449(2):202-7. doi: 10.1016/j.bbrc.2014.04.161. Epub 2014 May 9.

Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11.

Author information

1
Department of Medical Science, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
2
Department of Brain Science, DGIST, Daegu 711-873, Republic of Korea.
3
School of Biological Sciences, Seoul National University, Seoul 151-747, Republic of Korea.
4
Department of Life Science and College of Natural Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
5
Department of Life Science and College of Natural Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: tskim@ewha.ac.kr.

Abstract

Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11, and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively. However, the molecular mechanism of Flo8-mediated transcriptional activation remains largely elusive. By generating serial deletion constructs, we revealed here that a novel transcriptional activation domain on its extreme C-terminal region plays a crucial role in activating transcription. On the other hand, the N-terminal LisH motif of Flo8 appears to be required for its physical interaction with another transcriptional activator, Mss11, for their cooperative transcriptional regulation of the shared targets. Additionally, GST pull-down experiments uncovered that Flo8 and Mss11 can directly form either a heterodimer or a homodimer capable of binding to DNA, and we also showed that this formed complex of two activators interacts functionally and physically with the Swi/Snf complex. Collectively, our findings provide valuable clues for understanding the molecular mechanism of Flo8-mediated transcriptional control of multiple targets.

KEYWORDS:

FLO1; FLO11; Flo8; LisH motif; Mss11; Transcriptional activation

PMID:
24813990
DOI:
10.1016/j.bbrc.2014.04.161
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center