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J Mol Biol. 2014 Jul 15;426(14):2580-93. doi: 10.1016/j.jmb.2014.04.028. Epub 2014 May 9.

Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2.

Author information

1
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA.
2
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
3
The New York Structural Biology Center, 89 Convent Avenue, New York, NY 10027, USA.
4
Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY 10031, USA; The Graduate Center of the City University of New York, 365 Fifth Avenue, New York, NY 10016, USA. Electronic address: rghose@sci.ccny.cuny.edu.

Abstract

In bacteriophages of the cystovirus family, the polymerase complex (PX) encodes a 75-kDa RNA-directed RNA polymerase (P2) that transcribes the double-stranded RNA genome. Also a constituent of the PX is the essential protein P7 that, in addition to accelerating PX assembly and facilitating genome packaging, plays a regulatory role in transcription. Deletion of P7 from the PX leads to aberrant plus-strand synthesis suggesting its influence on the transcriptase activity of P2. Here, using solution NMR techniques and the P2 and P7 proteins from cystovirus ϕ12, we demonstrate their largely electrostatic interaction in vitro. Chemical shift perturbations on P7 in the presence of P2 suggest that this interaction involves the dynamic C-terminal tail of P7, more specifically an acidic cluster therein. Patterns of chemical shift changes induced on P2 by the P7 C-terminus resemble those seen in the presence of single-stranded RNA suggesting similarities in binding. This association between P2 and P7 reduces the affinity of the former toward template RNA and results in its decreased activity both in de novo RNA synthesis and in extending a short primer. Given the presence of C-terminal acidic tracts on all cystoviral P7 proteins, the electrostatic nature of the P2/P7 interaction is likely conserved within the family and could constitute a mechanism through which P7 regulates transcription in cystoviruses.

KEYWORDS:

NMR; RNA-directed RNA polymerase; cystovirus; polymerase complex; transcription

PMID:
24813120
PMCID:
PMC4090703
DOI:
10.1016/j.jmb.2014.04.028
[Indexed for MEDLINE]
Free PMC Article
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