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EMBO J. 2014 Jun 17;33(12):1321-40. doi: 10.1002/embj.201386917. Epub 2014 May 8.

Specific ablation of Nampt in adult neural stem cells recapitulates their functional defects during aging.

Author information

1
Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA.
2
Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, USA imaishin@wustl.edu.

Abstract

Neural stem/progenitor cell (NSPC) proliferation and self-renewal, as well as insult-induced differentiation, decrease markedly with age. The molecular mechanisms responsible for these declines remain unclear. Here, we show that levels of NAD(+) and nicotinamide phosphoribosyltransferase (Nampt), the rate-limiting enzyme in mammalian NAD(+) biosynthesis, decrease with age in the hippocampus. Ablation of Nampt in adult NSPCs reduced their pool and proliferation in vivo. The decrease in the NSPC pool during aging can be rescued by enhancing hippocampal NAD(+) levels. Nampt is the main source of NSPC NAD(+) levels and required for G1/S progression of the NSPC cell cycle. Nampt is also critical in oligodendrocytic lineage fate decisions through a mechanism mediated redundantly by Sirt1 and Sirt2. Ablation of Nampt in the adult NSPCs in vivo reduced NSPC-mediated oligodendrogenesis upon insult. These phenotypes recapitulate defects in NSPCs during aging, giving rise to the possibility that Nampt-mediated NAD(+) biosynthesis is a mediator of age-associated functional declines in NSPCs.

KEYWORDS:

Nampt; adult neural stem cells; aging; differentiation; proliferation

PMID:
24811750
PMCID:
PMC4194122
DOI:
10.1002/embj.201386917
[Indexed for MEDLINE]
Free PMC Article

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