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J Med Primatol. 2014 Oct;43(5):329-40. doi: 10.1111/jmp.12123. Epub 2014 May 9.

DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.

Author information

1
Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.

Abstract

BACKGROUND:

A desirable HIV vaccine should induce protective long-lasting humoral and cellular immune responses.

METHODS:

Macaques were immunized by env DNA, selected from a panel of recently transmitted SIVmac251 Env using intradermal electroporation as vaccine delivery method and magnitude, breadth and longevity of humoral and cellular immune responses.

RESULTS:

The macaques developed high, long-lasting humoral immune responses with neutralizing capacity against homologous and heterologous Env. The avidity of the antibody responses was also preserved over 1-year follow-up. Analysis of cellular immune responses demonstrated induction of Env-specific memory T cells harboring granzyme B, albeit their overall levels were low. Similar to the humoral responses, the cellular immunity was persistent over the ~1-year follow-up.

CONCLUSION:

These data show that vaccination by this intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.

KEYWORDS:

DNA vaccine; HIV; SIV; avidity; binding antibody; cellular responses; durability of responses; neutralizing antibody

PMID:
24810337
PMCID:
PMC4176517
DOI:
10.1111/jmp.12123
[Indexed for MEDLINE]
Free PMC Article

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