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Exp Neurol. 2014 Jul;257:114-9. doi: 10.1016/j.expneurol.2014.04.017. Epub 2014 May 5.

Intermittent fasting attenuates inflammasome activity in ischemic stroke.

Author information

1
Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
2
School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia.
3
School of Pharmacy, Sungkyunkwan University, Suwon, South Korea.
4
Department of Physiology, Georgia Health Sciences University, Augusta, GA, USA.
5
Laboratory of Neurosciences, National Institute on Aging, Intramural Research Program, Baltimore, MD, USA.
6
Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; School of Pharmacy, Sungkyunkwan University, Suwon, South Korea; School of Biomedical Sciences, The University of Queensland, St Lucia, Queensland, Australia. Electronic address: phstva@nus.edu.sg.

Abstract

Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16h of food deprivation daily) for 4months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.

KEYWORDS:

Inflammasomes; Intermittent fasting; Ischemic stroke; NLRP1; NLRP3

PMID:
24805069
DOI:
10.1016/j.expneurol.2014.04.017
[Indexed for MEDLINE]

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