Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement

Taku Satoh; Shinji Sugiura; Kimio Sumaru; Shigenori Ozaki; Shinichi Gomi; Tomoaki Kurakazu; Yasuhiro Oshima; Toshiyuki Kanamori

doi:10.1063/1.4871036

Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement

Biomicrofluidics. 2014 Apr 16;8(2):024112. doi: 10.1063/1.4871036. eCollection 2014 Mar.

Authors

Taku Satoh¹, Shinji Sugiura¹, Kimio Sumaru¹, Shigenori Ozaki², Shinichi Gomi², Tomoaki Kurakazu², Yasuhiro Oshima², Toshiyuki Kanamori¹

Affiliations

¹ Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
² Tokyo Electron Limited, 5-3-1 Akasaka, Minato-ku, Tokyo 107-6325, Japan.

Abstract

We present a novel cell culture chip, namely, "inverting microwell array chip," for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip.