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Bioorg Khim. 1989 Sep;15(9):1239-45.

[Cloning and determination of the primary structure of cDNA, coding the central part of elongation factor 2 from the rat liver].

[Article in Russian]


A number of cDNA clones coding for 417 amino acid residues of the central part of the rat liver elongation factor 2 (EF-2) have been isolated. The oligonucleotides complementary to EF-2 mRNA were used as primers for synthesis of the first strand of cDNA cloned. Structures of these oligonucleotides were determined in course of 3'----5' sequencing coding strand of EF-2 cDNA. This method of synthesis of specific cDNA enabled one to reduce essentially the number of recombinant clones to be screened for EF-2 cDNA. Comparative studies of deduced protein sequences of rat liver EF-2 and hamster EF-2 [1] revealed the only substitution of aspartate residue for glutamate residue (hamster EF-2). The homology between nucleotide sequences of rat and hamster EF-2 cDNA was 89%. Northern-blot analysis of rat liver poly(A)+ mRNA revealed the only species of mRNA 3000 nucleotides long. A strong stop-signal for reverse transcriptase in the 5'-region of rat liver EF-2 mRNA is discovered: probability of dissociation of the enzyme from mRNA is at least 97.5%.

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