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Biochem Biophys Res Commun. 1989 Nov 15;164(3):1316-22.

Isolation of a cDNA that hybrid selects antiproliferative mRNA from rat liver.

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S.R. Noble Foundation, Ardmore, OK 73402.


Studies of chromosome loss in inherited cancers, of fusions between proliferating and quiescent cells, and of microinjection of RNA from quiescent cells into proliferation competent cells have all provided evidence for antiproliferative genes in mammalian cells. In this report, we describe a partial cDNA clone isolated on the basis of its preferential hybridization to RNA from normal versus regenerating rat liver. The corresponding mRNA, enriched by hybrid selection, was microinjected into normal human diploid fibroblasts in cell culture, resulting in a 53% decrease in the fraction of nuclei incorporating tritiated thymidine. This mRNA is 2 kb in size and is expressed in eight tissues examined.

[Indexed for MEDLINE]

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