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J Neuroimmunol. 2014 Jul 15;272(1-2):56-61. doi: 10.1016/j.jneuroim.2014.04.005. Epub 2014 Apr 16.

Multiple sclerosis: the elevated antibody response to Epstein-Barr virus primarily targets, but is not confined to, the glycine-alanine repeat of Epstein-Barr nuclear antigen-1.

Author information

1
Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany. Electronic address: klemens.ruprecht@charite.de.
2
Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
3
JPT Peptide Technologies GmbH, Berlin, Germany.
4
Institute for Medical Immunology, Charité - Universitätsmedizin Berlin, Germany.
5
Institute of Medical Biometrics and Clinical Epidemiology, Charité - Universitätsmedizin Berlin, Germany.
6
Labor Berlin Charité-Vivantes GmbH, Berlin, Germany; Institute of Medical Virology, Charité - Universitätsmedizin Berlin, Germany.
7
Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany; NeuroCure Clinical Research Center, Charité - Universitätsmedizin Berlin, Germany.

Abstract

Patients with multiple sclerosis (MS) have elevated antibodies against Epstein-Barr virus (EBV), but data on the epitope-resolved specificity of these antibodies are scarce. Using a peptide microarray containing 1465 peptides representing 8 full-length EBV proteins, we identified higher (p<0.001) antibody reactivities to 39 EBV-peptides in MS patients (n=29) compared to healthy controls (n=22). Seventeen of the 39 peptides were from EBNA-1 and 13 located within the glycine-alanine repeat of EBNA-1. Further reactivities were directed against EBNA-3, EBNA-4, EBNA-6, VP26, and LMP1. Thus, antibodies against EBV in MS patients primarily target, but are not confined to, the glycine-alanine repeat of EBNA-1.

KEYWORDS:

Antibodies; Epstein–Barr nuclear antigen-1; Epstein–Barr virus; Glycine–alanine repeat; Multiple sclerosis; Peptide array

PMID:
24798244
DOI:
10.1016/j.jneuroim.2014.04.005
[Indexed for MEDLINE]

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