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J Virol. 1989 Dec;63(12):5489-91.

Inhibition of human immunodeficiency virus type 1 RNase H by sulfated polyanions.

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1
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.

Abstract

The reverse transcriptase (RT) activity of human immunodeficiency virus type 1 and other retroviruses is closely associated with a hybrid-degrading RNase H activity which is essential for retroviral replication. We have analyzed the effect of sulfated polysaccharides on human immunodeficiency virus type 1 recombinant RT and RNase H activities in vitro. Heparin, dextran sulfates, and xylan polysulfate were found to be much more potent inhibitors of RNase H than of RT and exhibit 50% infective doses of 0.04 to 0.1 micrograms/ml (corresponding to 0.1 to 25 nM) which is up to 5,000-fold more efficient than that for RT. Inhibitors of RNase H activity are attractive as antiviral drugs.

PMID:
2479774
PMCID:
PMC251222
[Indexed for MEDLINE]
Free PMC Article
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