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Dev Dyn. 2014 Aug;243(8):988-98. doi: 10.1002/dvdy.24143. Epub 2014 May 19.

A population of progenitor cells in the basal and intermediate layers of the murine bladder urothelium contributes to urothelial development and regeneration.

Author information

1
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin.

Abstract

BACKGROUND:

Homeostatic maintenance and repair of the bladder urothelium has been attributed to proliferation of keratin 5-expressing basal cells (K5-BC) with subsequent differentiation into superficial cells. Recent evidence, however, suggests that the intermediate cell layer harbors a population of progenitor cells. We use label-retaining cell (LRC) methodology in conjunction with a clinically relevant model of uropathogenic Escherichia coli (UPEC)-induced injury to characterize urothelial ontogeny during development and in response to diffuse urothelial injury.

RESULTS:

In the developing urothelium, proliferating cells were dispersed throughout the K5-BC and intermediate cells layers, becoming progressively concentrated in the K5-BC layer with age. When 5-bromo-2-deoxyuridine (BrdU) was administered during urothelial development, LRCs in the adult were found within the K5-BC, intermediate, and superficial cell layers, the location dependent upon time of labeling. UPEC inoculation resulted in loss of the superficial cell layer followed by robust proliferation of K5-BCs and intermediate cells. LRCs within the K5-BC and intermediate cell layers proliferated in response to injury.

CONCLUSIONS:

Urothelial development and regeneration following injury relies on proliferation of K5-BC and intermediate cells. The existence and proliferation of LRCs within both the K5-BC and intermediate cell layers suggests the presence of two populations of urothelial progenitor cells.

KEYWORDS:

bacteria; cystitis; progenitor; urinary injury

PMID:
24796293
PMCID:
PMC4111772
DOI:
10.1002/dvdy.24143
[Indexed for MEDLINE]
Free PMC Article

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