Format

Send to

Choose Destination
Osteoarthritis Cartilage. 2014 Jul;22(7):941-50. doi: 10.1016/j.joca.2014.04.028. Epub 2014 May 2.

Repetitive allogeneic intraarticular injections of synovial mesenchymal stem cells promote meniscus regeneration in a porcine massive meniscus defect model.

Author information

1
Department of Joint Surgery and Sports Medicine, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
2
Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
3
Department of Cartilage Regeneration, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
4
Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, Japan.
5
Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: sekiya.arm@tmd.ac.jp.

Abstract

OBJECTIVE:

A new strategy is required in order to regenerate a meniscus for extensive defects. Synovial mesenchymal stem cells (MSCs) are an attractive cell source for meniscus regeneration due to their high proliferation and chondrogenic potential. We examined the effect of repetitive intraarticular injections of synovial MSCs on meniscus regeneration in a massive meniscal defect of pigs. We followed up the efficacy using MRI evaluation in addition to macroscopic and histological observations.

DESIGN:

Two weeks before the injection of synovial MSCs, the anterior half of the medial menisci was resected in both knees of pigs. Fifty million allogeneic synovial MSCs were injected into the right knee at 0, 2, and 4 weeks and followed up by sequential MRI. The regenerated meniscus, adjacent articular cartilage, and subchondral bone were evaluated by MRI at 2, 4, 8, 12 and 16 weeks. They were also evaluated macroscopically and histologically at 16 weeks (n = 7).

RESULTS:

The resected meniscus regenerated significantly better in the MSC group than in the control group based on histological and MRI analyses. Macroscopically, the meniscal defect already appeared to be filled with synovial tissue at 2 weeks. Articular cartilage and subchondral bone at the medial femoral condyle were also significantly more preserved in the MSC group based on MRI, macroscopic, and histological analyses.

CONCLUSIONS:

Intraarticular injections of allogeneic synovial MSCs appeared to promote meniscus regeneration and provide protection at the medial femoral articular cartilage in a porcine massive meniscal defect model.

KEYWORDS:

Meniscus; Mesenchymal stem cells; Osteoarthritis; Pig; Regeneration; Synovium

PMID:
24795274
DOI:
10.1016/j.joca.2014.04.028
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center