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Mol Cell. 2014 May 22;54(4):613-25. doi: 10.1016/j.molcel.2014.03.043. Epub 2014 May 1.

A role for WDR5 in integrating threonine 11 phosphorylation to lysine 4 methylation on histone H3 during androgen signaling and in prostate cancer.

Author information

1
Division of Reproductive Science in Medicine, Department of OB/GYN, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
2
Division of Reproductive Science in Medicine, Department of OB/GYN, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Driskill Graduate Program, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
3
Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
4
Division of Endocrinology, Metabolism and Molecular Medicine, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
5
Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
6
Division of Endocrinology, Metabolism and Molecular Medicine, Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
7
Division of Reproductive Science in Medicine, Department of OB/GYN, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: debu@northwestern.edu.

Erratum in

  • Mol Cell. 2015 May 7;58(3):557.

Abstract

Upon androgen stimulation, PKN1-mediated histone H3 threonine 11 phosphorylation (H3T11P) promotes AR target gene activation. However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P, and this interaction facilitates the recruitment of the MLL1 complex and subsequent H3K4 tri-methylation (H3K4me3). Using ChIP-seq, we find that androgen stimulation results in a 6-fold increase in the number of H3T11P-marked regions and induces WDR5 colocalization to one third of H3T11P-enriched promoters, thus establishing a genome-wide relationship between H3T11P and recruitment of WDR5. Accordingly, PKN1 knockdown or chemical inhibition severely blocks WDR5 chromatin association and H3K4me3 on AR target genes. Finally, WDR5 is critical in prostate cancer cell proliferation and is hyperexpressed in human prostate cancers. Together, these results identify WDR5 as a critical epigenomic integrator of histone phosphorylation and methylation and as a major driver of androgen-dependent prostate cancer cell proliferation.

PMID:
24793694
PMCID:
PMC4075454
DOI:
10.1016/j.molcel.2014.03.043
[Indexed for MEDLINE]
Free PMC Article

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