Format

Send to

Choose Destination
Nat Methods. 2014 Jun;11(6):653-5. doi: 10.1038/nmeth.2960. Epub 2014 May 4.

Towards error-free profiling of immune repertoires.

Author information

1
1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3].
2
1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2].
3
1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
4
Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia.
5
Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
6
Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
7
1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia.
8
1] Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia. [2] Pirogov Russian National Research Medical University, Moscow, Russia. [3] Central European Institute of Technology, Masaryk University, Brno, Czech Republic.

Abstract

Deep profiling of antibody and T cell-receptor repertoires by means of high-throughput sequencing has become an attractive approach for adaptive immunity studies, but its power is substantially compromised by the accumulation of PCR and sequencing errors. Here we report MIGEC (molecular identifier groups-based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error correction while fully preserving the natural diversity of complex immune repertoires.

Comment in

PMID:
24793455
DOI:
10.1038/nmeth.2960
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center