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Curr Opin Chem Biol. 2014 Jun;20:22-8. doi: 10.1016/j.cbpa.2014.04.005. Epub 2014 May 8.

Quantitative super-resolution microscopy: pitfalls and strategies for image analysis.

Author information

1
ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, Av. Carl Friedrich Gauss, 3, 08860 Castelldefels (Barcelona), Spain.
2
ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, Av. Carl Friedrich Gauss, 3, 08860 Castelldefels (Barcelona), Spain. Electronic address: melike.lakadamyali@icfo.es.

Abstract

Super-resolution microscopy is an enabling technology that allows biologists to visualize cellular structures at nanometer length scales using far-field optics. To break the diffraction barrier, it is necessary to leverage the distinct molecular states of fluorescent probes. At the same time, the existence of these different molecular states and the photophysical properties of the fluorescent probes can complicate data quantification and interpretation. Here, we review the pitfalls in super-resolution data analysis that must be avoided for proper interpretation of images.

PMID:
24793374
DOI:
10.1016/j.cbpa.2014.04.005
[Indexed for MEDLINE]
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