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J Photochem Photobiol B. 2014 Jun 5;135:1-6. doi: 10.1016/j.jphotobiol.2014.04.008. Epub 2014 Apr 19.

Ultraviolet-B light induced oxidative stress: effects on antioxidant response of Spodoptera litura.

Author information

1
Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem 11, Tamil Nadu, India.
2
Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem 11, Tamil Nadu, India. Electronic address: skentomol@gmail.com.

Abstract

Ultraviolet light (UV-B), which emits radiation in the range of 280-315 nm, has been used worldwide in light trapping of insect pests. In this article, we test the hypothesis that one of the duration of UV-B exposure has a differential impact on oxidative stress marker enzymes in Spodoptera litura. Effect of UV-B exposure on total protein and antioxidant activities of superoxide dismutase (SOD), catalase (CAT), peroxidases (POX) and glutathione-S-transferase (GST) were investigated in S. litura. The adults were exposed to UV-B light for various time periods (0, 30, 60, 90 and 120 min). We found that exposure to UV-B light for 30 and 60 min resulted in increased activities of POX. When the exposure time lasted for 60 and 90 min, the activities of SOD remained significantly higher than the control. However, the POX, CAT and GST activity decreased to control levels at 90 and 120 min. whereas relatively long duration exposure activates the xenobiotics detoxifying enzymes like GST and POX and CAT enzymes. Longer UV-B exposure may interfere with pesticide detoxification mechanism in insects, making them more susceptible to insecticides.

KEYWORDS:

Antioxidant enzymes; Oxidative stress; Reactive oxygen species; Spodoptera litura; Ultraviolet light

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