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Osteoarthritis Cartilage. 2014 Oct;22(10):1396-400. doi: 10.1016/j.joca.2014.04.015. Epub 2014 Apr 30.

Quantitative X-ray microradiography for high-throughput phenotyping of osteoarthritis in mice.

Author information

1
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: j.waung@imperial.ac.uk.
2
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: s.maynard13@imperial.ac.uk.
3
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: s.gopal13@imperial.ac.uk.
4
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: a.gogakos@imperial.ac.uk.
5
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: j.logan@imperial.ac.uk.
6
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: graham.williams@imperial.ac.uk.
7
Molecular Endocrinology Group, Department of Medicine, Imperial College London, London W12 0NN, UK. Electronic address: d.bassett@imperial.ac.uk.

Abstract

OBJECTIVE:

To investigate and validate digital X-ray microradiography as a novel, high-throughput and cost-effective screening approach to identify abnormal joint phenotypes in mice.

METHOD:

Digital X-ray microradiography was used to quantify the subchondral bone mineral content (BMC) in the medial tibial plateau. Accuracy and reproducibility of the method were determined in 22 samples from C57BL/6(B6Brd;B6Dnk;B6N-Tyr(c-Brd)) wild-type mice. The method was then validated in wild-type mice that had undergone surgical destabilisation of medial meniscus (DMM) and in a genetically modified mouse strain with an established increase in trabecular bone mass.

RESULTS:

The measurement of subchondral BMC by digital X-ray microradiography had a coefficient of variation of 3.6%. Digital X-ray microradiography was able to demonstrate significantly increased subchondral BMC in the medial tibial plateau of male mice 4 and 8 weeks after DMM surgery and in female mice 8 weeks after surgery. Furthermore, digital X-ray microradiography also detected the increase in subchondral BMC in a genetically modified mouse strain with high trabecular bone mass.

CONCLUSION:

Quantitation of subchondral BMC by digital X-ray microradiography is a rapid, sensitive and cost-effective method to identify abnormal joint phenotypes in mice of both genders at several ages.

KEYWORDS:

Bone mineral content; Destabilisation of the medial meniscus; Digital X-ray microradiography; Mouse phenotyping; Osteoarthritis; Subchondral bone

PMID:
24792211
PMCID:
PMC4192136
DOI:
10.1016/j.joca.2014.04.015
[Indexed for MEDLINE]
Free PMC Article

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