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Methods Mol Biol. 2014;1156:447-63. doi: 10.1007/978-1-4939-0685-7_30.

Analysis of protein structure by cross-linking combined with mass spectrometry.

Author information

1
University of Victoria - Genome BC Proteomics Centre, University of Victoria, 3101-4464 Markham St, Victoria, BC, 78Z 7X8, Canada.

Abstract

Cross-linking combined with mass spectrometry is a powerful technique to study protein structure. Here, we present an optimized protocol for the preparation, processing, and analysis of a protein sample cross-linked with isotopically coded, affinity-enrichable, and CID-cleavable cross-linker CyanurBiotinDimercaptoPropionylSuccinimide using LC/ESI-MS/MS on a Thermo Scientific Orbitrap mass spectrometer.

PMID:
24792007
DOI:
10.1007/978-1-4939-0685-7_30
[Indexed for MEDLINE]

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