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Methods Mol Biol. 2014;1156:379-88. doi: 10.1007/978-1-4939-0685-7_25.

Quantitative glycoproteomics for N-glycoproteome profiling.

Author information

1
Department of Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA, 98195, USA, shengp@medicine.washington.edu.

Abstract

Glycoproteomics, the global analysis of glycoproteins, is a subfield of proteomics with high biological and clinical relevance. Glycoproteomics integrates knowledge and techniques drawing from glycotechnology, separation science, mass spectrometry, and bioinformatics to support systematic identification and quantification of glycoproteins in a complex biological sample. In this chapter, we describe a shotgun proteomics-based glycoproteomics platform that provides precise mapping of protein N-glycosylation sites and quantitative assessment of glycosylation site occupancy on a global scale.

PMID:
24792002
DOI:
10.1007/978-1-4939-0685-7_25
[Indexed for MEDLINE]

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