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Int J Nanomedicine. 2014 Apr 17;9:1931-46. doi: 10.2147/IJN.S59870. eCollection 2014.

Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo.

Author information

1
Department of Head and Neck, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming; Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming, The People's Republic of China.
2
Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan, The People's Republic of China ; The First People's Hospital of Yunnan Province (The Affiliated Hospital of Kunming University of Science and Technology), Kunming, Yunnan, The People's Republic of China.
3
Department of Head and Neck, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, The People's Republic of China.
4
Kunming Institute of Precious Metals, Kunming, Yunnan, The People's Republic of China.
5
Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan, The People's Republic of China.
6
Department of Head and Neck, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan, The People's Republic of China.
7
Institute of Oncology, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan, The People's Republic of China.
8
Clinical Skills Training Center of Kunming Medical University, Kunming, Yunnan, The People's Republic of China.
9
Department of Anesthesiology, Yan An Hospital, Kunming, Yunnan, The People's Republic of China.

Abstract

Gold nanorods (AuNRs) have been used in plasmonic photothermal therapy (PPTT), which is thought to be more efficient and selective than conventional photothermal therapy. The efficiency and safety of PPTT can be improved by functionally modifying the gold nanorods with proteins or biomolecules. In this study, AuNRs were modified with anti-epidermal growth factor receptor (EGFR) monoclonal antibody (mAb), and the apoptotic potential of EGFRmAb-AuNR was assessed in Hep-2 cells in vitro and in vivo. The EGFRmAb modification had no obvious influence on the original optical property of the AuNRs, but it significantly increased the entry of AuNRs into Hep-2 cells. EGFRmAb-AuNRs, with appropriate laser irradiation, resulted in higher Hep-2 cells apoptosis than AuNRs did alone, in vitro, and was accompanied by alteration of reactive oxygen species (ROS) production, Ca(2+) release, change in mitochondrial membrane potential (ΔΨm), cytochrome c (Cyt-c) release, active caspase-3 expression, and level of B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma 2 protein-associated X protein (Bax). EGFRmAb-AuNR-mediated apoptosis in Hep-2 cells was also observed in vivo and had an inhibitive effect on growth of Hep-2 tumor xenografts. Our data suggest that the EGFRmAb modification improves AuNR-mediated apoptosis and may have the potential to be used clinically.

KEYWORDS:

AuNRs; PPTT; laryngeal squamous cancer cells; plasmonic photothermal therapy

PMID:
24790435
PMCID:
PMC4000183
DOI:
10.2147/IJN.S59870
[Indexed for MEDLINE]
Free PMC Article
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