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Bioresour Technol. 2014 Jun;162:389-91. doi: 10.1016/j.biortech.2014.04.011. Epub 2014 Apr 16.

Accelerated glycerol fermentation in Escherichia coli using methanogenic formate consumption.

Author information

1
Institut für angewandte Biowissenschaften, Angewandte Biologie, Karlsruher Institut für Technologie, Fritz-Haber-Weg 2, D-76131 Karlsruhe, Germany.
2
Institut für angewandte Biowissenschaften, Angewandte Biologie, Karlsruher Institut für Technologie, Fritz-Haber-Weg 2, D-76131 Karlsruhe, Germany. Electronic address: johannes.gescher@kit.edu.

Abstract

Escherichia coli can ferment glycerol anaerobically only under very defined restrictive conditions. Hence, it was the aim of this study to overcome this limitation via a co-cultivation approach. Anaerobic glycerol fermentation by a pure E. coli culture was compared to a co-culture that also contained the formate-oxidizing methanogen Methanobacterium formicicum. Co-cultivation of the two strains led to a more than 11-fold increased glycerol consumption. Furthermore, it supported a constantly neutral pH and a shift from ethanol to succinate production. Moreover, M. formicicum was analyzed for its ability to grow on different standard media and a surprising versatility could be demonstrated.

KEYWORDS:

Co-culture; E. coli; Formate; Glycerol fermentation; M. formicicum

PMID:
24785787
DOI:
10.1016/j.biortech.2014.04.011
[Indexed for MEDLINE]

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