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Inflamm Res. 2014 Aug;63(8):657-65. doi: 10.1007/s00011-014-0738-0. Epub 2014 Apr 30.

Vespa tropica venom suppresses lipopolysaccharide-mediated secretion of pro-inflammatory cyto-chemokines by abrogating nuclear factor-κ B activation in microglia.

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1
National Brain Research Centre, Near NSG campus, Manesar-122050, Gurgaon, Haryana, 122051, India, deepakkaushiik@gmail.com.

Abstract

OBJECTIVE AND DESIGN:

The present study was aimed to evaluate the anti-inflammatory potentials of Vespa tropica (VT) venom and its isolated peptides. Effects of whole venom and its two peptides (Vt1512 and Vt1386) on lipopolysaccharide (LPS) challenged BV-2 murine microglial cells was evaluated.

MATERIALS:

Mouse microglial cell line, BV-2 and crude venom extract as well as purified peptides from VT along with LPS from Salmonella enterica were used for the studies.

TREATMENT:

BV-2 cells were treated with 500 ng/ml of LPS and different doses of crude wasp venom as well as purified peptides.

METHODS:

We used immunoblotting, cytokine bead arrays and fluorescence activated cell sorter (FACS) to evaluate the levels of various proteins, cytokines and reactive oxygen species (ROS).

RESULTS:

Our studies suggest that treatment with whole venom significantly reduces oxidative stress and LPS-stimulated activation of microglia. Also, purified peptides from crude venom exhibited potential anti-inflammatory properties. Further, whole venom was found to be targeting Akt and p38 MAPK pathways, leading to suppressed NF-κB phosphorylation in LPS challenged BV-2 cells.

CONCLUSIONS:

VT venom possesses anti-inflammatory properties and can be further explored for their therapeutic potential in treating various inflammatory conditions of the central nervous system (CNS).

PMID:
24781802
DOI:
10.1007/s00011-014-0738-0
[Indexed for MEDLINE]
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