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Methods. 2014 Aug 1;68(3):450-7. doi: 10.1016/j.ymeth.2014.04.015. Epub 2014 Apr 26.

Methodological considerations for heat shock of the nematode Caenorhabditis elegans.

Author information

1
Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA.
2
Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA. Electronic address: jly@alum.mit.edu.

Abstract

Stress response pathways share commonalities across many species, including humans, making heat shock experiments valuable tools for many biologists. The study of stress response in Caenorhabditis elegans has provided great insight into many complex pathways and diseases. Nevertheless, the heat shock/heat stress field does not have consensus as to the timing, temperature, or duration of the exposure and protocols differ extensively between laboratories. The lack of cohesiveness makes it difficult to compare results between groups or to know where to start when preparing your own protocol. We present a discussion of some of the major hurdles to reproducibility in heat shock experiments as well as detailed protocols for heat shock and hormesis experiments.

KEYWORDS:

Heat shock; Heat stress; Hormesis

PMID:
24780523
PMCID:
PMC4112136
DOI:
10.1016/j.ymeth.2014.04.015
[Indexed for MEDLINE]
Free PMC Article

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