Yessotoxin triggers ribotoxic stress

Toxicol In Vitro. 2014 Aug;28(5):975-81. doi: 10.1016/j.tiv.2014.04.013. Epub 2014 Apr 26.

Abstract

This work tests the hypothesis that the marine algal toxin yessotoxin (YTX) can trigger ribotoxic stress response in L6 and BC3H1 myoblast cells. YTX exposure at a concentration of 100 nM displays the characteristics of a ribotoxic stress response in such cells. The exposure leads to activation of the p38 mitogen-activated protein kinase, the stress-activated protein kinase c-jun, and the double-stranded RNA-activated protein kinase (PKR). YTX treatment also causes ribosomal RNA cleavage and inhibits protein synthesis. These observations support the idea that YTX can act as a ribotoxin.

Keywords: 28S rRNA cleavage; PKR; Protein synthesis inhibition; Ribotoxic stress response; Yessotoxin; c-jun.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Marine Toxins / toxicity*
  • Mice
  • Mollusk Venoms
  • Oxocins / toxicity*
  • Protein Biosynthesis / drug effects
  • RNA Cleavage
  • RNA, Ribosomal, 28S / metabolism
  • Rats
  • eIF-2 Kinase / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Marine Toxins
  • Mollusk Venoms
  • Oxocins
  • RNA, Ribosomal, 28S
  • eIF-2 Kinase
  • JNK Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • yessotoxin