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Worm. 2013 Jul 1;2(3):e25081. doi: 10.4161/worm.25081. Epub 2013 May 21.

Deep sequencing strategies for mapping and identifying mutations from genetic screens.

Author information

1
Institut de GA(c)nA(c)tique et de Biologie MolA(c)culaire et Cellulaire (IGBMC); Institut National de la SantA(c) et de la Recherche MA(c)dicale (INSERM) U964/Centre National de la Recherche Scientifique (CNRS) UMR 1704/UniversitA(c) de Strasbourg; Strasbourg, France.

Abstract

The development of next-generation sequencing technologies has enabled rapid and cost effective whole genome sequencing. This technology has allowed researchers to shortcut time-consuming and laborious methods used to identify nucleotide mutations in forward genetic screens in model organisms. However, causal mutations must still be mapped to a region of the genome so as to aid in their identification. This can be achieved simultaneously with deep sequencing through various methods. Here we discuss alternative deep sequencing strategies for simultaneously mapping and identifying causal mutations in Caenorhabditis elegans from mutagenesis screens. Focusing on practical considerations, such as the particular mutant phenotype obtained, this review aims to aid the reader in choosing which strategy to adopt to successfully clone their mutant.

KEYWORDS:

C. elegans; EMS; NGS; SNP mapping; WGS; deep sequencing; forward genetics; genetic mapping; next-generation sequencing; whole genome sequencing

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