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Anticancer Res. 2014 May;34(5):2165-71.

Overexpression of NOTCH-regulated ankyrin repeat protein is associated with breast cancer cell proliferation.

Author information

1
4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. t_imaoka@nirs.go.jp.

Abstract

BACKGROUND/AIM:

NOTCH-regulated ankyrin repeat protein (NRARP) has been implicated in crosstalk between NOTCH and wingless-type mouse mammary tumor virus integration site (WNT) signals during development. Our study aimed to clarify its role in breast cancer cells.

MATERIALS AND METHODS:

Public microarray data were used to analyze gene expression in human and rat breast cancer. A short interfering RNA was introduced into MCF7 and T47D human breast cancer cells for NRARP silencing. Gene expression was analyzed by quantitative polymerase chain reaction.

RESULTS:

The NRARP transcript was commonly overexpressed in various rat mammary cancer models. In addition, a subset of human breast cancer also expressed high levels of NRARP transcript, which correlated positively with up-regulation of cell proliferation-related genes. Silencing of NRARP suppressed the growth of MCF7 and T47D cells and lowered the expression of cell cycle-related genes in MCF7 cells.

CONCLUSION:

NRARP may stimulate cell proliferation in human breast cancer.

KEYWORDS:

NOTCH-regulated ankyrin repeat protein; NRARP; breast cancer

PMID:
24778018
[Indexed for MEDLINE]

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