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Antimicrob Agents Chemother. 2014 Jul;58(7):3921-6. doi: 10.1128/AAC.02489-13. Epub 2014 Apr 28.

Validation of antibiotic susceptibility testing guidelines in a routine clinical microbiology laboratory exemplifies general key challenges in setting clinical breakpoints.

Author information

1
Institut für Medizinische Mikrobiologie, Universität Zürich, Zürich, Switzerland mhombach@imm.uzh.ch.
2
Unite des Agents Antibactériens, Institut Pasteur, Paris, France.
3
Institut für Medizinische Mikrobiologie, Universität Zürich, Zürich, Switzerland.

Abstract

This study critically evaluated the new European Committee for Antimicrobial Susceptibility Testing (EUCAST) antibiotic susceptibility testing guidelines on the basis of a large set of disk diffusion diameters determined for clinical isolates. We report several paradigmatic problems that illustrate key issues in the selection of clinical susceptibility breakpoints, which are of general importance not only for EUCAST but for all guidelines systems, i.e., (i) the need for species-specific determinations of clinical breakpoints/epidemiological cutoffs (ECOFFs), (ii) problems arising from pooling data from various sources, and (iii) the importance of the antibiotic disk content for separating non-wild-type and wild-type populations.

PMID:
24777093
PMCID:
PMC4068562
DOI:
10.1128/AAC.02489-13
[Indexed for MEDLINE]
Free PMC Article

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