Format

Send to

Choose Destination
J Stroke Cerebrovasc Dis. 2014 Jul;23(6):1396-402. doi: 10.1016/j.jstrokecerebrovasdis.2013.11.021. Epub 2014 Apr 26.

Activation of peroxisome proliferator-activated receptor β/δ attenuates acute ischemic stroke on middle cerebral ischemia occlusion in rats.

Author information

1
Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, China; Department of Neurosurgery, Urumqi General Hospital of PLA, Urumqi, China.
2
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
3
Department of Cardiology, The First Hospital of PLA, Lanzhou, China.
4
Department of Neurosurgery, Xijing Institute of Clinical Neuroscience, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
5
Department of Neurosurgery, People's Hospital of Jishan County, Shanxi, China.
6
Department of Neurosurgery, Xinjiang Corps Hospital, Urumqi, China.
7
Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, China. Electronic address: kelyue@msn.com.

Abstract

BACKGROUND:

Peroxisome proliferator-activated receptor (PPAR)-β/δ is a transcription factor that belongs to the nuclear hormone receptor family. There is little information about the effects of the immediate administration of specific ligands of PPAR-β/δ (GW0742) in animal models of acute ischemic stroke. Using a rat model of middle cerebral ischemia occlusion (MCAO) in vivo, we have investigated the effect of pretreatment with GW0742 before MCAO.

METHODS:

The neuroprotective effect of GW0742 against acute ischemic stroke was evaluated by the neurologic deficit score (NDS), dry-wet weight, and 2,3,5-triphenyltetrazolium chloride staining. The levels of interleukin (IL)-1β, nuclear factor (NF)-κB, and tumor necrosis factor (TNF)-α were detected by an enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS), Bax, and Bcl-2 were detected by Western blot. The apoptotic cells were counted by in situ terminal deoxyribonucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay.

RESULTS:

The pretreatment with GW0742 significantly increased the expression of Bcl-2, and significantly decreased in the volume of infarction, NDS, edema, expressions of IL-1β, NF-κB, TNFα, and Bax, contents of iNOS and the apoptotic cells in infarct cerebral hemisphere compared with rats in the vehicle group at 24 hours after MCAO.

CONCLUSIONS:

The study suggests the neuroprotective effect of the PPAR-β/δ ligand GW0742 in acute ischemic stroke by a mechanism that may involve its anti-inflammatory and antiapoptotic action.

KEYWORDS:

GW0742; PPAR-β/δ; anti-inflammatory; middle cerebral artery occlusion (MCAO); rat

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center