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J Diabetes Res. 2014;2014:743798. doi: 10.1155/2014/743798. Epub 2014 Mar 17.

Tracing fasting glucose fluxes with unstressed catheter approach in streptozotocin induced diabetic rats.

Author information

1
Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
2
Department of Endocrinology, Zhejiang Province People's Hospital, Hangzhou 310014, China.

Abstract

OBJECTIVE:

Blood glucose concentrations of type 1 diabetic rats are vulnerable, especially to stress and trauma. The present study aimed to investigate the fasting endogenous glucose production and skeletal muscle glucose uptake of Streptozotocin induced type 1 diabetic rats using an unstressed vein and artery implantation of catheters at the tails of the rats as a platform.

RESEARCH DESIGN AND METHODS:

Streptozotocin (65 mg·kg⁻¹) was administered to induce type 1 diabetic state. The unstressed approach of catheters of vein and artery at the tails of the rats was established before the isotope tracer injection. Dynamic measurement of fasting endogenous glucose production was assessed by continuously infusing stable isotope [6, 6-²H₂] glucose, while skeletal muscle glucose uptake by bolus injecting radioactively labeled [1-¹⁴C]-2-deoxy-glucose.

RESULTS:

Streptozotocin induced type 1 diabetic rats displayed polydipsia, polyphagia, and polyuria along with overt hyperglycemia and hypoinsulinemia. They also had enhanced fasting endogenous glucose production and reduced glucose uptake in skeletal muscle compared to nondiabetic rats.

CONCLUSIONS:

The dual catheters implantation at the tails of the rats together with isotope tracers injection is a save time, unstressed, and feasible approach to explore the glucose metabolism in animal models in vivo.

PMID:
24772449
PMCID:
PMC3977083
DOI:
10.1155/2014/743798
[Indexed for MEDLINE]
Free PMC Article

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