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Front Neural Circuits. 2014 Apr 3;8:29. doi: 10.3389/fncir.2014.00029. eCollection 2014.

Simultaneous imaging of neural activity in three dimensions.

Author information

1
Department of Biological Sciences, Columbia University New York, NY, USA.

Abstract

We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.

KEYWORDS:

brain activity map; calcium imaging; spatial-light-modulator; three-dimensional imaging; volume imaging

PMID:
24772066
PMCID:
PMC3982072
DOI:
10.3389/fncir.2014.00029
[Indexed for MEDLINE]
Free PMC Article

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