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J Pathol. 2014 Aug;233(4):331-343. doi: 10.1002/path.4360. Epub 2014 May 27.

Mixed-species biofilm compromises wound healing by disrupting epidermal barrier function.

Author information

1
Comprehensive Wound Center, Davis Heart & Lung Research Institute, Centers for Regenerative Medicine and Cell Based Therapies, The Ohio State University, Columbus, OH 43220 USA.
2
Department of Surgery, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
3
Microbial Interface Biology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
4
Department of Microbial Infection and Immunity, Microbiology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
5
Deparment of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
6
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
7
Department of Materials Science and Engineering, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
8
Department of Plastic Surgery, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43220 USA.
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Contributed equally

Abstract

In chronic wounds, biofilm infects host tissue for extended periods of time. This work establishes the first chronic preclinical model of wound biofilm infection aimed at addressing the long-term host response. Although biofilm-infected wounds did not show marked differences in wound closure, the repaired skin demonstrated compromised barrier function. This observation is clinically significant, because it leads to the notion that even if a biofilm infected wound is closed, as observed visually, it may be complicated by the presence of failed skin, which is likely to be infected and/or further complicated postclosure. Study of the underlying mechanisms recognized for the first time biofilm-inducible miR-146a and miR-106b in the host skin wound-edge tissue. These miRs silenced ZO-1 and ZO-2 to compromise tight junction function, resulting in leaky skin as measured by transepidermal water loss (TEWL). Intervention strategies aimed at inhibiting biofilm-inducible miRNAs may be productive in restoring the barrier function of host skin.

KEYWORDS:

microRNA; mixed-species biofilm; porcine burn wounds; transepidermal water loss (TEWL); wound biofilm

PMID:
24771509
PMCID:
PMC4380277
DOI:
10.1002/path.4360
[Indexed for MEDLINE]
Free PMC Article

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