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Clin Infect Dis. 2014 Jul 15;59(2):272-8. doi: 10.1093/cid/ciu292. Epub 2014 Apr 24.

Emerging technologies for rapid identification of bloodstream pathogens.

Author information

1
Division of Infectious Diseases, VA Greater Los Angeles Healthcare System.
2
Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center.
3
Division of Infectious Diseases, VA Greater Los Angeles Healthcare System Departments of Medicine, Urology, and Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, California.

Abstract

Technologies for rapid microbial identification are poised to revolutionize clinical microbiology and enable informed decision making for patients with life-threatening bloodstream infections. Species identification of microorganisms in positive blood cultures can be performed in minutes using commercial fluorescence in situ hybridization tests or mass spectroscopy. Microorganisms in positive blood cultures can also be identified within 1-2.5 hours using automated polymerase chain reaction-based systems that can also detect selected antibiotic resistance markers, such as methicillin resistance. When combined with antibiotic stewardship programs, these approaches improve clinical outcomes and reduce healthcare expenditures. Tests for direct detection in whole blood samples are highly desirable because of their potential to identify bloodstream pathogens without waiting 1-2 days for blood cultures to become positive. However, results for pathogen detection in whole blood do not overlap with those of conventional blood culture techniques and we are still learning how best to use these approaches.

KEYWORDS:

antimicrobial stewardship; bloodstream infections; clinical microbiology; molecular diagnostics; rapid diagnostics

PMID:
24771332
PMCID:
PMC4368854
DOI:
10.1093/cid/ciu292
[Indexed for MEDLINE]
Free PMC Article

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