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Sci Rep. 2014 Apr 28;4:4738. doi: 10.1038/srep04738.

Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa.

Author information

1
1] Department of Microbiology, Third Military Medical University, Chongqing, 400038, China [2] School of Dentistry, University of California, Los Angeles, CA, 90095, USA.
2
Department of Microbiology, Third Military Medical University, Chongqing, 400038, China.
3
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
4
School of Dentistry, University of California, Los Angeles, CA, 90095, USA.

Abstract

Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation. In this study, Pseudomonas aeruginosa PA1 strain was infected with lytic phage PaP1, and phage-resistant mutants were selected. A high percentage (~30%) of these mutants displayed red pigmentation phenotype (Red mutant). Through comparative genomic analysis, one Red mutant PA1r was found to have a 219.6 kb genomic fragment deletion, which contains two key genes hmgA and galU related to the observed phenotypes. Deletion of hmgA resulted in the accumulation of a red compound homogentisic acid; while A galU mutant is devoid of O-antigen, which is required for phage adsorption. Intriguingly, while the loss of galU conferred phage resistance, it significantly attenuated PA1r in a mouse infection experiment. Our study revealed a novel phage resistance mechanism via chromosomal DNA deletion in P. aeruginosa.

PMID:
24770387
PMCID:
PMC4001099
DOI:
10.1038/srep04738
[Indexed for MEDLINE]
Free PMC Article

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