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Biosens Bioelectron. 2014 Oct 15;60:71-6. doi: 10.1016/j.bios.2014.03.069. Epub 2014 Apr 8.

Preparation of surface-imprinted polymer grafted with water-compatible external layer via RAFT precipitation polymerization for highly selective and sensitive electrochemical determination of brucine.

Author information

1
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China.
2
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei Province, PR China. Electronic address: bzzeng@whu.edu.cn.

Abstract

A novel brucine imprinted polymer was prepared on multi-walled carbon nanotubes by reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization. The polymer was further grafted with hydrophilic poly(glycerol monomethacrylate) brushes to improve its water-compatibility. The obtained molecularly imprinted material showed enhanced accessibility to brucine and improved selective recognition property in water medium. When the material was supported on an ionic liquid functionalized graphene coated glassy carbon electrode for the electrochemical determination of brucine, the resulting electrochemical sensor presented good analytical performance. Under the optimized conditions, the peak current was linear to brucine concentration in the ranges of 0.006-0.6 μM and 0.6-5.0 μM with sensitivities of 15.3 μA/μMmm(2) and 5.4 μA/μM mm(2), respectively; the detection limit was 2 nM (S/N=3). The sensor was successfully applied to the determination of brucine in practical samples and the recovery for the standards added was 94-104%.

KEYWORDS:

Brucine; Electrochemical sensor; Molecularly imprinted polymer; Poly(glycerol monomethacrylate); RAFT polymerization

PMID:
24769450
DOI:
10.1016/j.bios.2014.03.069
[Indexed for MEDLINE]

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