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Neurosci Lett. 2014 Jun 6;570:102-7. doi: 10.1016/j.neulet.2014.04.020. Epub 2014 Apr 24.

Motor cortex glutathione deficit in ALS measured in vivo with the J-editing technique.

Author information

1
Department of Radiology, Weill Cornell Medical College, 516 East 72nd Street, New York, NY 10021, United States.
2
Department of Neurology, Columbia University, 710 West 168th Street, New York, NY 10032, United States.
3
Department of Neurology, Hospital of Special Surgery, 525 East 71st Street, New York, NY 10021, United States.
4
Department of Radiology, Weill Cornell Medical College, 516 East 72nd Street, New York, NY 10021, United States. Electronic address: dcs7001@med.cornell.edu.

Abstract

This study compared in vivo levels of the antioxidant glutathione (GSH) in the motor cortex of 11 ALS patients with those in 11 age-matched healthy volunteers (HV). Using the standard J-edited spin-echo difference MRS technique, GSH spectra were recorded on a 3.0 T GE MR system from a single precentral gyrus voxel. GSH levels expressed as ratios to the unsuppressed voxel tissue water (W) were 31% lower in ALS patients than in HV (p=.005), and 36% lower in ALS than in HV (p=.02) when expressed as ratios to the total creatine peak (tCr), supporting a role for oxidative stress in ALS. Levels of the putative neuronal marker N-acetylaspartate (NAA) relative to W did not differ between ALS and HV (p=.26), but were lower by 9% in ALS than in HV (p=.013) when expressed as ratios relative to tCr. This discrepancy is attributed to small but opposite changes in NAA and tCr in ALS that, as a ratio, resulted in a statistically significant group difference, further suggesting caution in using tCr as an internal reference under pathological conditions.

KEYWORDS:

Amyotrophic lateral sclerosis; Biomarker; Glutathione; Magnetic resonance spectroscopy; Neurodegeneration; Oxidative stress

PMID:
24769125
DOI:
10.1016/j.neulet.2014.04.020
[Indexed for MEDLINE]
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