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Methods. 2014 Aug 1;68(3):441-9. doi: 10.1016/j.ymeth.2014.04.011. Epub 2014 Apr 21.

Methodological considerations for mutagen exposure in C. elegans.

Author information

1
Magee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, United States.
2
Magee-Womens Research Institute, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, United States. Electronic address: yanowitzjl@mwri.magee.edu.

Abstract

Maintenance of the genome requires the continual repair of DNA lesions. Exposure of nematodes to DNA damage-inducing agents is a powerful method to rapidly ascribe a role for specific genes in DNA repair and to define epistatic relationships to other repair genes which allows for the construction of repair pathways. Despite the extensive use of these agents, however, differences in dosing, timing, and handling makes it difficult to compare results across laboratories. We provide herein a consideration of the parameters that influence the results of these exposures and detailed protocols for the exposure to mutagenic inducing agents.

KEYWORDS:

DNA damage repair; Double-strand breaks; Mutagen

PMID:
24768858
PMCID:
PMC5449201
DOI:
10.1016/j.ymeth.2014.04.011
[Indexed for MEDLINE]
Free PMC Article

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