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J Ethnopharmacol. 2014 Jun 11;154(2):471-4. doi: 10.1016/j.jep.2014.04.015. Epub 2014 Apr 23.

Antitubercular constituents from Premna odorata Blanco.

Author information

1
Graduate School, University of Santo Tomas, España, Manila 1015, Philippines.
2
Graduate School, University of Santo Tomas, España, Manila 1015, Philippines; Phytochemistry Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila 1015, Philippines.
3
Phytochemistry Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila 1015, Philippines.
4
Institut fur Organische Chemie, Universitat Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany.
5
Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street, Chicago, IL 60612-7231, USA.
6
Graduate School, University of Santo Tomas, España, Manila 1015, Philippines; Phytochemistry Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila 1015, Philippines. Electronic address: alicia.aguinaldo@gmail.com.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Premna odorata Blanco (Lamiaceae) is a medicinal plant traditionally used in Albay Province, in southeastern Luzon, Philippines to treat tuberculosis. This study aimed to determine the antitubercular property of the crude extract and sub-extracts of the leaves, and to isolate the bioactive principles from the active fractions.

MATERIALS AND METHODS:

Through extraction, solvent polarity-based fractionation and silica gel chromatography purification of the DCM sub-extract, compound mixtures from the bioactive fractions were isolated and screened for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv using the colorimetric Microplate Alamar Blue assay (MABA).

RESULTS:

The crude methanolic extract and sub-extracts showed poor inhibitory activity against Mycobacterium tuberculosis H37Rv (MIC≥128µg/mL). However, increased inhibitory potency was observed for fractions eluted from the DCM sub-extract (MIC=54 to 120µg/mL). Further purification of the most active fraction (MIC=54µg/mL) led to the isolation of a 1-heneicosyl formate (1), 4:1 mixture of β-sitosterol (2), stigmasterol (3) and diosmetin (4), which were identified through GC-MS analysis (with dereplication) and NMR experiments. The MIC of compound 1 was 8µg/mL.

CONCLUSIONS:

The results of this study provide scientific basis for the traditional use of Premna odorata as treatment for tuberculosis.

KEYWORDS:

1-heneicosyl formate; Antitubercular; Diosmetin; Premna odorata; Tuberculosis

PMID:
24768632
DOI:
10.1016/j.jep.2014.04.015
[Indexed for MEDLINE]

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