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Exp Mol Pathol. 2014 Jun;96(3):422-5. doi: 10.1016/j.yexmp.2014.04.009. Epub 2014 Apr 21.

Elevated circulating miR-150 and miR-342-3p in patients with irritable bowel syndrome.

Author information

1
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States.
2
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States; Howard Hughes Medical Institute National Institutes of Health Research Scholar, Chevy Chase, MD 20815, United States.
3
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States; National Institute on Minority Health and Health Disparities, National Institutes of Health, DHHS, Bethesda, MD 20892, United States.
4
Internal Medicine, Medical School, University of Michigan, Ann Arbor, MI 48109, United States.
5
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States. Electronic address: hendersw@mail.nih.gov.

Abstract

BACKGROUND AND AIMS:

MicroRNAs (miRNAs) are small non-coding RNAs, which regulate gene expression and are thus of interest as diagnostic markers, and as clues to etiology and targets of intervention. This pilot study examined whether circulating miRNAs are differentially expressed in patients with IBS.

METHODS:

miRNA microarrays (NanoString) were run on the whole blood of 43 participants.

RESULTS:

hsa-miR-150 and hsa-miR-342-3p were found to be significantly elevated (FDR adjusted p≤0.05, ≥1.6 fold change) in IBS patients compared to healthy controls. Neither of these miRNAs showed any relationship to race or sex. hsa-miR-150 is associated with inflammatory bowel disorders and pain, and interacts with a protein kinase (AKT2) through which it may affect inflammatory pathways. hsa-miR-342-3p is predicted to interact with mRNAs involved in pain signaling, colonic motility, and smooth muscle function.

CONCLUSIONS:

This preliminary study reports the association of two miRNAs, detected in whole blood, with IBS. These miRNAs link to pain and inflammatory pathways both of which are thought to be dysregulated in IBS. Larger sample sizes are needed to confirm their importance and potential as biomarkers.

KEYWORDS:

Irritable bowel syndrome; MicroRNA; hsa-miR-342-3p; hsa-miRNA-150

PMID:
24768587
PMCID:
PMC4119883
DOI:
10.1016/j.yexmp.2014.04.009
[Indexed for MEDLINE]
Free PMC Article

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